Xenograft Brain Tumor Models
Xenograft models have emerged as pivotal tools in neuro-oncology, especially for the study of glioblastoma (GBM). The ability of xenografts to mimic human tumor biology makes them indispensable for preclinical studies. At Alfa Cytology, we specialize in providing comprehensive preclinical research services, including the development and utilization of xenograft brain tumor models.
Introduction to Xenograft Brain Tumor Models
Xenograft brain tumor models are characterized by the implantation of human-derived tumor cells into immunodeficient mice, such as NOD-scid or athymic nude mice. These models facilitate the examination of tumor growth dynamics, metastatic behavior, and therapeutic efficacy in a controlled environment that closely resembles human physiology. The advantages of using xenograft models include:
Realistic Tumor Microenvironment
Xenografts maintain the heterogeneity and complexity of human tumors, offering insights into tumor-host interactions and the tumor microenvironment (TME).
Therapeutic Evaluation
These models are widely used to assess the efficacy of new drugs, radiation therapies, and immunotherapies. They provide critical data on how human tumors respond to various treatment modalities.
Genetic Manipulation
Researchers can genetically modify tumor cells to study specific oncogenic pathways, facilitating the investigation of targeted therapies.
Our Services
At Alfa Cytology, we specialize in providing comprehensive preclinical research services, including the development and utilization of xenograft brain tumor models. Our state-of-the-art facilities and expert team enable us to deliver high-quality research tailored to the specific needs of our clients.
PDX Model
Available Brain Tumor PDX Model
- GBM-PDX-1
- GBM-PDX-2
- GBM-PDX-3
- MB-PDX
CDX Model
Available Brain Tumor CDX Model
- Glioblastoma: M059J, U87MG, U251, A172, LN-229, U-118MG
- Neuroblastoma: SH-SY5Y
- Medulloblastoma: DAOY, D283
- Astrocytoma: U373
- Ependymoma: CHLA-266
Case Study - GL261-Luc Xenograft Model
Model Introduction
The GL261-Luc xenograft model provides a well-established preclinical platform for studying glioma biology and evaluating novel therapeutic strategies. GL261 is a murine glioma cell line derived from a glioblastoma tumor induced in C57BL/6 mice. This model is widely used in cancer research due to its immunocompetent host background, allowing for the investigation of tumor-immune interactions and the evaluation of immunotherapies.
Model Information
- Model: GL261-Luc Xenograft Model
- Animals: C57BL/6 Mice
- Age: 6 Weeks
- Cancer Type: Glioma
- Weight: 18-20 g
Model Construction
The model is established by orthotopically implanting GL261-Luc murine glioma cells into the brain of immunocompetent C57BL/6 mice. Cell inoculum is 8×105 cells/mouse. Tumor growth is monitored non-invasively using bioluminescent imaging, enabling real-time tracking of intracranial tumor progression and body weight monitoring.
Fig. 1 Workflow of IOMM-Lee xenograft model establishment. (Source: Alfa Cytology)
Model Data
- Tumorigenicity: GL261-Luc cells demonstrate consistent and reproducible tumor formation in C57BL/6 mice, with stable bioluminescent signal detectable post-implantation and high engraftment rates.
- Growth Kinetics: Tumor growth is progressive and measurable via bioluminescent imaging. Bioluminescent signal increases steadily over time, providing a defined window for therapeutic intervention. Body weight remains stable during early tumor growth, with minimal impact on overall health.
Fig. 2 Representative bioluminescent imaging and tumor growth curves of GL261-Luc orthotopic xenograft model. Data are presented as mean ± standard error (SEM). (Source: Alfa Cytology)
Contact Us
For researchers and organizations seeking to advance their understanding of brain tumors through xenograft models, Alfa Cytology offers unparalleled expertise and resources. Our dedicated team is ready to assist you in your preclinical research endeavors. To discuss your specific needs or to learn more about our services, please feel free to contact us to customize your needs.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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